As a physical phenomenon, achieving and controlling a large anomalous Hall effect (AHE) is crucial both for fundamental research and industrial applications. The recently discovered topological Kagome metal AV3Sb5 (A = K, Rb, Cs) exhibits a significant AHE along with charge density wave (CDW) and superconductivity, providing an ideal platform to study the interactions between nontrivial band topology, CDW, and superconductivity. In this study, we systematically investigated the evolution of AHE, CDW and superconductivity in electron (Mn)-doped Cs(V1−xMnx)3Sb5 single crystals. The experimental results show that electron doping rapidly suppresses superconductivity, while the CDW order remains relatively robust. Meanwhile, a significantly enhanced AHE, with a maximum anomalous Hall conductivity (AHC) of ∼25331 Ω-1cm-1 and an anomalous Hall angle of ∼6.66% occurs at a relatively low doping level of x = 0.03. Based on the Tian–Ye–Jin (TYJ) scaling model, such a significant enhancement of AHC is mainly dominated by the skew scattering. We speculated the enhanced skew scattering from conduction electrons of Mn originates from the strengthened spin-orbital coupling. Our finding provides important guidance for the design and development of transverse transport properties in topological Kagome materials.